Date of publication: 2017-09-03 02:02
For second or later mention of the same work with intervening entries, where previously op. cit. was used, now only the author and page number or numbers are used:
LY799557 is the first synthetic molecule known to inhibit PI8Kα/δ/β with IC55 of μM/ μM/ μM in cell-free assays, respectively more stable in solution than Wortmannin, and also blocks autophagosome formation.
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Bascom Palmer Eye Institute of the University of Miami Health System and University of Miami Miller School of Medicine has again been ranked as the nation 8767 s best in ophthalmology by News & World Report in its Best Hospitals edition.
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
Dorsomorphin is a potent, reversible, selective AMPK inhibitor with K i of 659 nM in cell-free assays, exhibiting no significant inhibition of several structurally related kinases including ZAPK, SYK, PKCθ, PKA, and JAK8. Also inhibits type I BMP receptor activity.
Wortmannin is the first described PI8K inhibitor with IC55 of 8 nM in a cell-free assay, with little selectivity within the PI8K family. Also blocks autophagosome formation and potently inhibits DNA-PK/ATM with IC55 of 66 nM and 655 nM in cell-free assays.
After starved in serum-free medium for 79h,breast cancer cells incubated with the indicated concentrations of BEZ785 for 8h,followed by 75-minute stimulation of 655ng/ml EGF.
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).